Qumica, FCT-UNL, 2829-516 Caparica, transfer of horseradish peroxidase (HRP) in plant monooxygenase (pMMO) is located at Site D in the protein structure Unlike the general concepts, it is including neuroleptics, antidepressants, b blockers, and N Nagoya 467-8603, Japan. erythropolis N771. These results suggest that copperdioxygen complex was formed by condition. University of Torino, Torino 10125, Italy. cyanide with HasA. Akadeemia tee 15, 12618 Tallinn, Estonia. In Both Mo1 atoms are raised above the basal plane comprising from the Ni2+ column chromatography than the regular His6-tag the heme pocket of recombinant human TDO (rhTDO) in ferric form likely to coordinate two [4Fe4S] clusters. Department of Chemistry, Graduate School of Science Osaka Osaka University, Toyonaka, Osaka 560-0043, Japan, SO42-, respectively. 2Departments of Biochemistry and Chemistry, College of Medicine, latanoprost) and carbonic anhydrase (CA) inhibitors are Cytochrome C552 is a highly thermostable c-type heme protein from Shusuke Yamanaka3, Mitsutaka Okumura2, Kizashi Yamaguchi3 Through the use of ESI-MS, it has now been shown that reactivity has remained to be clarified. immobilization of Ami on the electrode. inhibitors for CA. catalytic action of the complexes were investigated by using the X-ray absorption spectra (XAS) of PAz variants and plastocyanins ZnPPSA was synthesized via seven steps and ZnMbSA insights into the metal binding mode of chaperons involved in the was migrated from aqueous to ionic liquid phase (Fig. successfully carried out by biocatalysis in the hydrated ILs. distributed on the plasma membrane in horseradish cell, thus La(III) Tohoku University, Sendai 980-8577, Japan, Bacterial assisted the maturation of NHase. clusters per dimer. second sphere of the active site. ligands. mixed solvents. 1Centre for Blood Research, University of British Columbia, deg -100 redox-dependent change of the ionization state of heme 17-propionic acid side Im Neuenheimer Feld 270, 69120 Heidelberg, Germany. an oxyferrous starting oxidation state in vivo. Taiwan, is calculated to be about 64 mV for FeS HN H-bonds, correlating Ori-ISP consists of 23 has been determined and sequence alignments performed. D60K, E85K, and neutralization of the heme propionates. and M(Pone)- have been obtained for M u Fe, Mn and Zn. IC is Institute for Molecular Science and Okazaki Institute for Integrative corresponding amides. contains one heme group. These ionic liquids showed full miscibility with water upon P548 are few studies about the enzymatic biodegradation of TCP by HRP, In addition, with Bioscience, Hosei University, Koganei 184-8584, Japan. Thus, we assumed that P14K interacted with NHase a subunit and CueO is a multicopper oxidase involved in the Cu efflux system of The latter zinc ion converted to bacteriochlorin ring by the sequential reductions of two were altered to Ala and Ala/Ser/Cys, respectively. 1. (EPR/HF-EPR) and Raman spectroscopies. reliable kinetic characterization. contact in the deep inside of the heme pocket. P573 These Nanodiscstabilized Dcytb is being used for kinetic and spectroscopic 3Institute of Environmental Microbiology, Kyowa Kako Co., Tokyo [3]. of atmospheric dinitrogen to ammonia. stepped conformation effectively distorted by the chirality of the Tokyo 113-8657, Japan. In human, one of the most REQUIMTE, Departamento de Qumica, Centro de Qumica Fina e mammalians, there was found a correlation between high PAP levels and which was found out to be more stable than a non-collinear state Here, we report pH dependence of structure and one solvent-exposed cysteine residue, Cys84, which is in close 3NOVOZYMES SPAIN S.A., Madrid, Spain. If you do not see its contents 1Department of Chemistry, National Chung Hsing University, mutating the Cys2His2-type to the His4-type (Sp1(F2H4)) in the determined the crystal structure of peroxo Mb at 1.20 A resolution characterized by the AFM technique. study, we replaced bArg90 and cArg136 of SCNase, which were 3Department of Materials Engineering, Nagaoka College of [MoIVO(DMED)2]2- (Chart 1) shows Conditions (KYOKUGEN), Osaka University, Osaka 560-8531, [1] has demonstrated the importance of structure on the distance H. Yoshikawa2, A. Suzuki3, Y. Yamamoto1 Conditions (KYOKUGEN), Osaka University, Osaka 560-8531, copper complexes. type of metal-TTF complex in which TTF is functionalized with 3NOVOZYMES SPAIN S.A., Madrid, Spain. oxygen species, and so on. 2The Key Laboratory of Industrial Biotechnology, Ministry of immobilization methods of enzymes are required for individual redox The samples were characterized by means of Shin-ichi Ozaki, Akira Nakahara, Chihori Sakaguchi catalytic center [1]. 1Graduate School of Materials Science, Nara Institute of Science and of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan, 2Research School of Chemistry, Australian National University, interested in understanding the O2 activation chemistry of these enzymes, An average hydroxylation at the terminal carbon of medium-chain-length alkanes The relationship between the oxidase residues around the heme. The results showed that addition of cationic information available for the protein, functional properties of the Ha. affinity of Mb. Ansari AZ, Bradner JE, OHalloran TV (1995) DNA-bend modulation in a repressor-to-activator switching mechanism. active site of the enzyme. covalently depending on conditions. model for investigating the structure-function relationship in This ready accessibility of the high-valent iron form of heme Using the pKa value as a quantitative measure of the effect of the uniexponential biodegradation in 30 min of 85 lM IC, which are 20 nM necessary also in the thylakoid lumen. Additionally, in order to quantitatively assess the effects of the CF3 that 1 may adopt a more chirally distorted conformation also in solution. mechanism is crucial. The effect of the Cys residue of Mb on the reactions with various cyts c display a greater range of reduction potentials. Department of Biotechnology and Life Science, Tokyo University to retain Zn2+ homeostasis. understand the role of the metal ions in catalysis. especially with reference to the stability of bound oxygen, we both enthalpy (DH) and entropy (DS) contributions. the 4-pyds/Au electrode, and the redox potential is 260 mV versus 6-positions are substrates, and monosubstituted hydroquinones are Although the heme environmental effects type I Cu center. nucleases involved in DNA degradation in Caenorhabditis elegans. Metallocenters of these components have been largely crystal structures in apo-form, Mn2+-bound active form and mimics both of reactivity and structure of the oxotransferases. reported previously. 1REQUIMTE, CQFB, Dep. H N O The catalytic oxidation current was observed and 2Research School Of Chemistry, The Australian National University, Japan suggested that the atomic species of the bridging ligand should be an characterised using EPR, MCD and a recently developed kinetic assay [1]. antiferromagnetically coupled (AF) state. nitriles to the amides, is industrially important because it is used for the heavy metal ions, we genetically inserted one to three amino acid A mutant of a bacterial enzyme from the thermophile Geobacillus The globular, Hiromi Togashi1, Junko Yano2, Vittal Yachandra2, The compound is can grow similarly as the cells transformed with wild type hpn gene Enzo Laurenti1, Tatiana Marchis1, Andrea Barbero1 Amyloid-b peptide (Ab) was the principal constituent of plaques and 2Present address: Department of Genetics in Ecology, University of Despite substantial difference in the shape and orientational

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